Anatomical features of some bones of the forelimbs of lions (Panthera leo) / Características anatómicas de algunos huesos de los miembros torácicos de leones (Panthera leo)

SUMMARY: We studied the bones of forelimb of four adult lions (Panthera leo) of both sexes to record the gross anatomical and morphometrical features of the scapula, humerus, radius and ulna. We observed some unique anatomical features that will be helpful for radiographic interpretation and forensic investigations. The lateral surface of scapula was unequally divided into supraspinous (fossa supraspinata) and infraspinous fossa (fossa infraspinata) by a well developed spine (spina scapulae). The acromion process was subdivided into suprahamate process (processus suprahamatus)and hamate process (processus hamatus); the later one was over hanged the glenoid cavity (cavitas glenoidalis), but the supraglenoid tubercle (tuberculum supraglenoidalis) was absent. The shaft (diaphysis) of humerus was compressed craniocaudally in proximal part, rounded to oval in middle part and compressed mediolaterally in distal part. A long, narrow supracondyloid foramen was found at distal limb just above the medial epicondyle (epicondylus medialis) which didn't connect the radial fossa (fossa radialis) with the olecranon fossa (fossa olecrani). The radius and ulna were twin bones where radius was articulated craniolateral to the ulna proximally and craniomedial to the ulna distally. However, the ulna was the longest bone in the forelimb of lion. The olecranon tuberosity of this bone had three prominences - two were cranially, whereas the caudal one was the largest and rounded. Distally projected styloid processes (processus styloideus) were found in the distal limb of both radius and ulna.

RESUMEN: Estudiamos los huesos de las miembros torácicos de cuatro leones adultos (Panthera leo) de ambos sexos para registrar las características anatómicas y morfométricas macroscópicas de la escápula, el húmero, el radio y la ulna. Se observaron algunas características anatómicas únicas que serán útiles para la interpretación radiográfica y las investigaciones forenses. La superficie lateral de la escápula se dividió de manera desigual en fosa supraespinosa y fosa infraspinosa por una columna bien desarrollada (espina de la escápula). El proceso del acromion se subdividió en proceso suprahamato (processus suprahamatus) y proceso hamato (processus hamatus); el tubérculo supraglenoideo (tuberculum supraglenoidalis) estaba ausente. La diáfisis (diafisis) del húmero estaba comprimida craneocaudalmente en la parte proximal, redondeada a ovalada en la parte media y comprimida mediolateralmente en la parte distal. Se encontró un foramen supracondileo largo y estrecho en la extremidad distal, por encima del epicóndilo medial (epicondylus medilaris) que no conectaba la fosa radial (fosa radial) con la fosa olecraneana (fossa olecrani). El radio y la ulna eran huesos idénticos en los que el radio se articulaba craneolateral a la ulna proximalmente, y craneomedial a la ulna distalmente. Sin embargo, la ulna era el hueso más largo del miembro torácico del león. La tuberosidad del olécranon de este hueso tenía tres prominencias: dos eran craneales, mientras que la caudal era la más grande y redondeada. Se encontraron procesos estiloides proyectados distalmente (processus styloideus) en la extremidades distales del radio y la ulna.

Clinical and morphological justification of the treatment of purulonecrotic lesion in deep structures of limb distal segment in pig: case report / [Justificativa clínica e morfológica do tratamento de lesão purulonecrótica em estruturas profundas do segmento distal de membros em porco: relato de caso]

Surgical pathology of the musculoskeletal system, and in particular the diseases of the limb distal segment in pigs are quite common. Their significant spread leads to economic losses due to culling, compulsory slaughter, short-received pig production and pig crop. The purpose of this work was to reveal clinical and morphological features of limb distal segment diseases in pigs and to study the possibility of preserving their health and productivity. The conservative treatment of purulonecrotic lesions in the deep structures of the limb distal segment in pigs is not promising. It is shown that the best way to treat a given pathology is amputation of a sick limb. The technique of carrying out exarticulation of talus shin consists in separation of soft tissues, capsule and ligaments, ligation of vessels, formation of stump. The postoperative recovery period of the animal body is 25 days.(AU)

A patologia cirúrgica do sistema musculoesquelético e, em particular, as doenças do segmento distal dos membros em suínos são bastante comuns. A sua propagação significativa leva a perdas econômicas devido ao abate seletivo, abate obrigatório, produção de suínos pouco recebida e colheita de suínos. O objetivo deste trabalho foi revelar características clínicas e morfológicas das doenças do segmento distal de membros em suínos e estudar a possibilidade de preservar sua saúde e produtividade. O tratamento conservador das lesões purulonecróticas nas estruturas profundas do segmento distal do membro em porcos não é promissor. Fica estabelecido que a melhor forma de tratar uma determinada patologia é a amputação de um membro doente. A técnica de realizar a exarticulação da canela do talos consiste na separação dos tecidos moles, cápsula e ligamentos, ligadura dos vasos, formação do coto. O período de recuperação pós-operatória do corpo do animal é de 25 dias.(AU)

Anatomia óssea e muscular do antebraço e mão de Chrysocyon brachyurus (Carnivora, Canidae) / Bone and muscular anatomy of the antibone and handof Chrysocyon brachyurus (Carnivora, Canidae)

O lobo-guará Chrysocyon brachyurus Illiger, 1815, é o maior canídeo da América do Sul, pesa cerca de 25 kg quando adulto e está ameaçado de extinção. Descrições anatômicas contribuem para a complementação das informações sobre espécies silvestres e para implicações conservacionistas, clínicas e cirúrgicas. Objetivou-se descrever os ossos e os músculos do antebraço e mão do lobo-guará. A preparação das peças foi feita a partir dos métodos usuais de dissecação, em animais preservados em solução de formol a 10%. Os espécimes pertencem ao acervo didático do Laboratório de Ensino e Pesquisa em Animais Silvestres da UFU e são provenientes de indivíduos atropelados. Os ossos descritos foram: rádio, ulna, ossos cárpico acessório, cárpico ulnar e cárpico intermédio; ossos cárpicos I, II, III e IV; ossos metacárpicos I, II, III, IV, V; falanges proximais, falanges médias e falanges distais do primeiro ao quinto dedo. Os músculos observados foram: extensor radial do carpo; pronador redondo; braquiorradial; extensor comum dos dedos; extensor ulnar do carpo; extensor lateral dos dedos; supinador; abdutor longo do dedo I; flexor radial do carpo; flexor profundo dos dedos; flexor superficial dos dedos; flexor ulnar do carpo; pronador quadrado; interflexor; lumbricais; abdutor curto dos dedos I e II e flexor curto do dedo I.

The maned wolf Chrysocyon brachyurus (Illiger, 1815) is the largest canid in South America, weighs about 25 kg as an adult and is threatened of extinction. Anatomical descriptions contribute to the complementation of information on wild species and for conservation, clinical and surgical implications. The purpose of this study was to describe the bones and muscles of the forearm and hand of the maned wolf. The methodology was through the usual dissecting methods in animals preserved in 10% formalin solution. The animals belong to the didactic collection of the Laboratory of Teaching and Research in Wild Animals of the UFU and come from run over. The bones evaluated were: radius, ulna, carpal accessory, carpi ulnar and carpi intermedium; carpal bones I, II, III and IV; metacarpal bones I, II, III, IV, V; proximal phalanges, middle phalanges and distal phalanges from first to fifth finger. The muscles observed were: radial extensor carpal; pronator round; brachioradial; common extensor of fingers; ulnar carpal extensor; lateral extensor of the fingers; supinator; abductor long finger I; flexor carpi radialis; flexor deep fingers; superficial flexor of the fingers; ulnar flexor of the carpus; square pronator; interflexor; lumbrils; short abductor of fingers I and II and short flexor of finger I.

Platelet rich plasma in arthroscopically repaired rotator cuff muscle: A meta-analysis of randomized controlled trials

Background@#Platelet-rich plasma (PRP) has been used as a biologic augmenter in arthroscopically repaired rotator cuff muscle. The objective of this meta-analysis is to compare the clinical and structural outcomes of patients with and without PRP supplementation in arthroscopic rotator cuff repair.@*Methods @#A systematic search in different online databases was done to evaluate studies involving PRP supplementation in arthroscopically repaired rotator cuff muscle, reviewing the re-tear rates, pain scale and functional shoulder scores in groups with and without PRP supplementation.@*Results@#Six studies were eligible for the meta-analysis. Arthroscopically repaired rotator cuff with PRP supplementation showed statistically better clinical and structural outcomes compared to the group who did not receive supplementation.@*Conclusion@#PRP as a biologic augmenter can decrease the re-tear rates as well as pain scale and can improve the functional shoulder scores of patients with arthroscopically repaired rotator cuff muscle.

Genetic Ablation of EWS RNA Binding Protein 1 (EWSR1) Leads to Neuroanatomical Changes and Motor Dysfunction in Mice

A recent study reveals that missense mutations of EWSR1 are associated with neurodegenerative disorders such as amyotrophic lateral sclerosis, but the function of wild-type (WT) EWSR1 in the central nervous system (CNS) is not known yet. Herein, we investigated the neuroanatomical and motor function changes in Ewsr1 knock out (KO) mice. First, we quantified neuronal nucleus size in the motor cortex, dorsal striatum and hippocampus of three different groups: WT, heterozygous Ewsr1 KO (+/−), and homozygous Ewsr1 KO (−/−) mice. The neuronal nucleus size was significantly smaller in the motor cortex and striatum of homozygous Ewsr1 KO (−/−) mice than that of WT. In addition, in the hippocampus, the neuronal nucleus size was significantly smaller in both heterozygous Ewsr1 KO (+/−) and homozygous Ewsr1 KO (−/−) mice. We then assessed motor function of Ewsr1 KO (−/−) and WT mice by a tail suspension test. Both forelimb and hindlimb movements were significantly increased in Ewsr1 KO (−/−) mice. Lastly, we performed immunohistochemistry to examine the expression of TH, DARPP-32, and phosphorylated (p)-DARPP-32 (Thr75) in the striatum and substantia nigra, which are associated with dopaminergic signaling. The immunoreactivity of TH and DARPP-32 was decreased in Ewsr1 KO (−/−) mice. Together, our results suggest that EWSR1 plays a significant role in neuronal morphology, dopaminergic signaling pathways, and motor function in the CNS of mice.

Osteología del miembro torácico de la corzuela parda Mazama gouazoubira (g. Fischer, 1814) (Cetartiodactyla: Cervidae) / Osteology of the thoracic limb of south american gray brocket Mazama gouazoubira (Fischer, 1814) (Cetartiodactyla: Cervidae)

El objetivo del estudio fue describir las características anatómicas y establecer hipótesis morfo-funcionales del esqueleto del miembro torácico de Mazama gouazoubira. Para ello, se utilizaron los miembros torácicos de cuatro adultos jóvenes. Los huesos se prepararon mediante ebullición y fueron blanqueados en una solución de peróxido de hidrógeno para obtener descripciones comparativas, mediciones osteométricas y radiografías digitales. En comparación con los rumiantes domésticos, la escápula resultó ser ancha y plana, con el acromion pequeño, el proceso coracoides muy pequeño y sin tuberosidad de la espina. El húmero se observó alargado con la diáfisis redondeada en una sección transversal y con proyecciones no articulares lisas. La ulna se unió al radio hasta la epífisis distal y juntos formaron un espacio interóseo proximal alargado y estrecho, sin surco vascular. El esqueleto de la mano presentó los metacarpianos III y IV bien desarrollados y fusionados, mientras que los metacarpianos II y V resultaron rudimentarios con disposición telemetacarpiana, filogenéticamente típico de cérvidos del Nuevo Mundo. Se encontraron cuatro dedos con tres falanges en cada uno, de los cuales, dos corresponden a los principales (III y IV) que llegan al suelo y dos son rudimentarios (II y V). Las radiografías nos permitieron visualizar los patrones de tensiones trabeculares normales y la osteometría permitió establecer relaciones con fines comparativos. Se reconocieron las adaptaciones esqueléticas del miembro torácico para favorecer la locomoción cursorial saltatoria.

This study aimed to describe anatomical features of the forelimb skeleton of Mazama gouazoubira in order to establish morphofunctional activity. Forelimbs of four young adult specimens were used to this end. The bones were prepared by boiling and cleared in a solution of hydrogen peroxide for comparative descriptions, osteometrics measurements and digital radiographs. Compared to domestic ruminants, the scapula proved to be wide and flat, with a small acromion, reduced coracoid process and lacking of spine tuberosity. The humerus had an elongated shaft with a rounded cross-section and discrete non-articular projections. The ulna accompanied the radio to the distal epiphysis and formed a long and narrow, proximally placed interosseous space without a vascular groove. The skeleton of the hand presented the rudimentary metacarpals II and V and the well-developed III and IV fused metacarpals, which is a typical telemetacarpal arrangement of close phylogenetically deer species from the New World. There were four fingers, each one with three phalanges; two main (III and IV) touching the ground and two rudimentary (II and V). Radiographs allowed visualizing patterns of normal trabecular tensions and osteometrics enabled to establish ratios for comparative purposes. Adaptations of skeletal forelimb to favor cursorial saltatory locomotion were typically identified.

Comparative kinematic gait analysis in young and old Beagle dogs

Age-related involution in dogs involves loss of muscle mass and changes in connective tissue and articular cartilage. The aim of this study was to examine whether an age-related influence on joint mobility can be detected in the absence of disease. Five young (mean age 2.0 years) and five old (mean age 10.4 years) healthy and sound Beagle dogs underwent computer-assisted gait analysis during locomotion on a treadmill. Shoulder, elbow, carpal, hip, stifle, and tarsal joint angles including joint angle progression curves, minimum and maximum joint angles, and range of motion (ROM) in degrees were analyzed. The old group had a smaller maximum joint angle (p = 0.037) and ROM (p = 0.037) of the carpal joint; there were similar tendencies in the shoulder, elbow, and carpal joints. Descriptive analysis of the progression curves revealed less flexion and extension of the forelimb joints. The results indicate restricted joint mobility of the forelimb in old dogs, primarily of the carpal joint. Results in the joints of the hindlimb were inconsistent, and the contrasting alterations may be due to a compensatory mechanism. As most alterations were found in the distal joints, these should receive particular attention when examining elderly dogs.

Reconstrucción funcional y estética del codo (a propósito de un caso) / Functional and aesthetic reconstruction of the elbow (apropos of a case)

La reconstrucción de la región periolécranon es compleja, ya que requiere tejidos que tengan suficiente elasticidad y resistencia y que permitan una rápida recuperación. Cuando la etiología es tumoral, se debe tener en cuenta la exéresis completa de la lesión, la conservación de la funcionalidad del miembro y el aspecto estético, respetando ese orden. Presentamos la evolución de una paciente con un sarcoma de partes blandas en la región del codo que, tras su extirpación en bloque, se reconstruyó con un colgajo pediculado braquial lateral de flujo inverso, y que posteriormente, para mejorar su aspecto estético, se realizó lipoaspiración del colgajo y relleno graso de la zona dadora

The reconstruction of the peri-olecranon region is complex, requiring tissues with enough elasticity and strength, to ensure a rapid recovery. When the etiology is tumoral, complete excision of the lesion, the mobility of the limb, and the aesthetics should be taken into account, in that order. We present the evolution of a patient with soft tissue sarcoma in the elbow region, which was removed, and the elbow was reconstructed with a lateral brachial pedicle flap withreverse flow. Afterwards, to improve their aesthetic appearance, liposuction of the flap was performed, filling the donor site with fat.

Ultrasound and gross anatomy of the brachial plexus and major nerves of the forelimb. An anesthetic approach using the domestic rabbit (Oyctolagus cuniculus) as an experimental model1

PURPOSE: To update the gross and sonographic anatomy and propose landmarks to perform ultrasound-guided (US-guided) axillary brachial plexus block (BPB) in rabbits. METHODS: Forty New Zeeland's rabbit (NZR) cadavers were dissected and the nerves were trimmed, identified, measured, and photographed. Additionally, in twenty NZRs, sonographic images of brachial plexus (BP) were performed through a simple-resolution ultrasound device. The US-guided block was achieved through a minimum volume of lidocaine necessary to surround the BP roots. The effectiveness of the brachial plexus block was assessed on sensitivity and motor functions. RESULTS: The BP resulted from connections between the ventral branches of the last four cervical spinal nerves and the first thoracic spinal nerve. In the axillary sonoanatomy, the BP appeared as an agglomerate of small, round hypoechoic structures surrounded by a thin hyperechoic ring. The amount of time and the minimum volume required to perform was 4.3 ± 2.3 min and 0.8 ± 0.3ml, respectively. CONCLUSIONS: The gross and sonographic anatomy of the BP showed uncommon morphological variations. Moreover, from sonographic landmarks, we showed complete reproducibility of the axillary US-guided brachial plexus block with simple resolution equipment and small volume of anesthetics required.

Functional Recovery Not Correlated with Axon Regeneration through Olfactory Ensheathing Cell-Seeded Scaffolds in a Model of Acute Spinal Cord Injury

The implantation of bioengineered scaffolds into lesion-induced gaps of the spinal cord is a promising strategy for promoting functional tissue repair because it can be combined with other intervention strategies. Our previous investigations showed that functional improvement following the implantation of a longitudinally microstructured collagen scaffold into unilateral mid-cervical spinal cord resection injuries of adult Lewis rats was associated with only poor axon regeneration within the scaffold. In an attempt to improve graft-host integration as well as functional recovery, scaffolds were seeded with highly enriched populations of syngeneic, olfactory bulb-derived ensheathing cells (OECs) prior to implantation into the same lesion model. Regenerating neurofilament-positive axons closely followed the trajectory of the donor OECs, as well as that of the migrating host cells within the scaffold. However, there was only a trend for increased numbers of regenerating axons above that supported by non-seeded scaffolds or in the untreated lesions. Nonetheless, significant functional recovery in skilled forelimb motor function was observed following the implantation of both seeded and non-seeded scaffolds which could not be correlated to the extent of axon regeneration within the scaffold. Mechanisms other than simple bridging of axon regeneration across the lesion must be responsible for the improved motor function.

Palmitoyl Serotonin Inhibits L-dopa-induced Abnormal Involuntary Movements in the Mouse Parkinson Model

L-3,4-dihydroxyphenylalanine (L-DOPA) is the most common treatment for patients with Parkinson's disease (PD). However, long term use of L-DOPA for PD therapy lead to abnormal involuntary movements (AIMs) known as dyskinesia. Fatty acid amide hydrolase (FAAH) is enriched protein in basal ganglia, and inhibition of the protein reduces dyskinetic behavior of mice. Palmitoyl serotonin (PA-5HT) is a hybrid molecule patterned after arachidonoyl serotonin, antagonist of FAAH. However, the effect of PA-5HT on L-DOPA-induced dyskinesia (LID) in PD have not yet been elucidated. To investigate whether PA-5HT relieve LID in PD and decrease hyperactivation of dopamine D1 receptors, we used the 6-hydroxydopomine (6-OHDA)-lesioned mouse model of PD and treated the L-DOPA (20 mg/kg) for 10 days with PA-5HT (0.3 mg/kg/day). The number of wall contacts with the forelimb in the cylinder test was significantly decreased by 6-OHDA lesion in mice and the pharmacotherapeutic effect of L-DOPA was also revealed in PA-5HT-treated mice. Moreover, in AIMs test, PA-5HT-treated mice showed significant reduction of locomotive, axial, limb, and orofacial AIMs score compared to the vehicle-treated mice. LID-induced hyper-phosphorylation of ERK1/2 and overexpression of FosB/ΔFosB was markedly decreased in 6-OHDA-lesioned striatum of PA-5HT-treated mice, indicating that PA-5HT decreased the dopamine D1 receptor-hyperactivation induced by chronic treatment of L-DOPA in dopamine-denervated striatum. These results suggest that PA-5HT effectively attenuates the development of LID and enhance of ERK1/2 phosphorylation and FosB/ΔFosB expression in the hemi-parkinsonian mouse model. PA-5HT may have beneficial effect on the LID in PD.

Características planimétricas y estereológicas de los nervios palmar lateral y medial en manos de equinos con y sin síndrome navicular / Planimetric and stereological characteristics of lateral and medial palmar nerves in hands of equine with and without navicular syndrome

El Síndrome Navicular (SN) es una de las patologías claudicógenas mas frecuentes en la práctica clínica equina. Se ha descrito, cierta susceptibilidad individual respecto a ésta presentación clínica, es decir, algunos equinos con SN claudican y otros, con similares hallazgos radiográficos en mano no lo hacen sugiriendo que existen diferencias cuantitativas, entre equinos con y sin SN, en las características de las fibras nerviosas de los nervios periféricos que inervan la mano. El objetivo del estudio fue describir las características morfológicas cuantitativas de los nervios palmar lateral y medial en equinos con SN. Se obtuvo manos mediante un muestreo dirigido y diagnosticadas por inspección visual y evaluación radiográfica. Se conformó dos grupos: "No afectadas" (n= 6) y "Síndrome Navicular" (n= 8). Para determinar diferencias cuantitativos entre ambos grupos se realizó: i) Estudio 1, evaluó las características morfológicas, planimétricas y estereológicas de los nervios palmar lateral y medial, ii) Estudio 2: evaluó la cantidad de axones mielinizados y no mielinizados. En ambos estudios, el análisis de los datos morfológicos, estereológicos y planimétricos, en general, no detectó diferencias significativas entre grupos. En conclusión, los resultados obtenidos en el estudio no entregan evidencia morfológica respecto a diferencias entre nervios palmares entre equinos con y sin SN.

Navicular Syndrome (NS) is one of the most frequent claudicogen pathologies in the equine clinical practice. Certain individual susceptibility has been described in this clinical presentation, that is, some horses with SN halt, while others with similar radiographic findings in hand do not, suggesting that there are quantitative differences, between horses with and without clinical presentation of SN, on the characteristics of the nerve fibers of the peripheral nerves of the hand. The aim of this study was to describe the morphologic quantitative characteristics of the lateral and medial palmar in horses with SN nerves. Hands were obtained by sampling directed and diagnosed by visual inspection and radiographic evaluation. Two groups were formed: "Not affected" (n= 6) and "Navicular Syndrome" (n = 8). To determine quantitative differences between two groups we performed: i) Study 1: evaluated the morphological, planimetric and stereological lateral and medial palmar nerves, ii) Study 2: evaluated the number of unmyelinated and myelinated axons. In both studies, analysis of stereological, morphological and planimetric data generally detected no significant differences between groups. In conclusion, the results of the study do not provide morphological evidence for differences between palmar nerves between horses with and without SN.

Descripción anatómica del músculo extensor del I y II dedo de la mano del zorro perruno (Cerdocyon thous Linnaeus, 1766) / Anatomical description of crab-eating fox' (Cerdocyon thous Linnaeus, 1766) extensor of digit i and ii muscle

El zorro perruno (Cerdocyon thous) es el cánido silvestre más común en América del Sur, con una relativa estabilidad poblacional, pero susceptible de ser atropellado y caer en trampas, pudiendo sufrir traumas en sus miembros torácicos, por lo tanto se requiere un conocimiento anatómico específico de sus músculos para intervenciones médicas y quirúrgicas. El objetivo principal de esta investigación fue realizar una descripción anatómica del músculo extensor del I y II dedo del C thous, en la cual, se reporta forma, origen, inserción, inervación e irrigación. Se diseccionaron de superficial a profundo los dos antebrazos de seis especímenes muertos donados por CORPOCALDAS a la Universidad de Caldas. El músculo extensor del I y II dedo en este estudio presentó características anatómicas similares a las reportadas a otras especies pero con una distribución tendinosa variante formando desde tres a cuatro ramas que se distribuyen del I al III dedo, incluso faltando a veces para el III dedo, pero de igual forma presenta una distribución que debe ser conocida para procedimientos que requieran de su conocimiento.

The crab-eating fox (Cerdocyon thous) is the most common wild canid in South America. With a relative demographic stability, this animal is susceptible of being hit and falling into traps, and can suffer trauma in its thoracic limbs; therefore a specific anatomical knowledge about its muscles is necessary for medical and surgical procedures. The main aim of this research was to describe the anatomy of the extensor muscle of digit I and II of the C. thous reporting form, origin, insertion, innervation and irrigation. The forearms of six donated dead specimens from CORPOCALDAS to Caldas University were dissected from superficial to deep. In this research, the extensor muscle of digit I and II showed similar anatomical features to those reported for other species but with a variant tendinous distribution that forms from three to four branches distributed from digit I to III. Although sometimes this distribution does not exist for the digit III; this similar distribution must be reported for procedures which require this knowledge.

Comparison of three skin-stretching devices for closing skin defects on the limbs of dogs

Our objective was to evaluate the effectiveness of skin-stretching devices for closing defects on the extremities of dogs. Antebrachial skin defects were created on the limbs of 24 dogs randomly divided into three groups. Skin stretchers included staples and sutures passing through them (group A), sutures and hypodermic needles (group B), and Pavletic device (group C). Wounds on the left were further undermined in all groups. Tension and blood perfusion were assessed. After removing the stretchers on day 3, the defects were sutured and wound healing was clinically scored. Histological variables evaluated were cellular infiltration, edema, collagen orientation, and thickness of epidermis. Significant differences in tension were found among groups (p < 0.0005) and between measurement times for undermined (p = 0.001) or non-undermined (p < 0.0005) wounds. In contrast, blood perfusion values did not differ significantly. Clinical scores for group B seemed to be better than those for groups A and C, but differences were not significant. Primary wound closure using the Pavletic device was not feasible. No significant differences in histological variables were found between groups. Skin stretching with staples or hypodermic needles resulted in successful wound management with minor side effects on skin histology and circulation.

Comparison of teratogenecity induced by nano- and micro-sized particles of zinc oxide in cultured mouse embryos / 대한수의학회지

The increasing uses of zinc oxide nanoparticles (nZnO) in industrial and personal care products raise possible danger of using nZnO in human. To determine whether ZnO induces size-dependent anomalies during embryonic organogenesis, mouse embryos on embryonic day 8.5 were cultured for 2 days under 50, 100, and 150 microg of nZnO (< 100 nm) or micro-sized ZnO (mZnO; 80 +/- 25 microm), after which the morphological changes, cumulative quantity of Zn particles, and expressions of antioxidant and apoptotic genes were investigated. Although embryos exposed to 50 microg of ZnO exhibited no defects on organogenesis, embryos exposed to over 100 microg of ZnO showed increasing anomalies. Embryos treated with 150 microg of nZnO revealed significant changes in Zn absorption level and morphological parameters including yolk sac diameter, head length, flexion, hindbrain, forebrain, branchial bars, maxillary process, mandibular process, forelimb, and total score compared to the same dose of mZnO-treated embryos. Furthermore, CuZn-superoxide dismutase, cytoplasmic glutathione peroxidase (GPx) and phospholipid hydroperoxidase GPx mRNA levels were significantly decreased, but caspase-3 mRNA level was greatly increased in nZnO-treated embryos as compared to normal control embryos. These findings indicate that nZnO has severer teratogenic effects than mZnO in developing embryos.

Estudo histológico e histoquímico da pele de jurará Kinosternon scorpioides scorpioides (Testudines: Kinosternidae) / Histological and histochemical study of the skin of the muçuã Kinosternon scorpioides scorpioides (Testudines: Kinosternidae)

O objetivo do presente trabalho foi analisar histológica e histo- químicamente a pele do jurará (Kinosternon scorpioides scorpioides). Foram utilizados seis animais (três machos e três fêmeas). Os animais foram eutanasiados com dose letal de tiopental sódico a 2,5%, para colheita de fragmentos de pele mole das patas e pescoço do animal, que após a fixação em líquido de Bouin, foram incluídos em parafina e corados pelas técnicas de hematoxilina-eosina, Giemsa, Sirius red, Reticulina de Gomori e Fucsina-resorcina de Weigert. Os resultados revelaram que a pele do jurará é delgada e composta de epiderme e derme. A epiderme é formada por estrato germinativo constituído por uma única camada de células cilíndricas; estrato espinhoso apresentando duas ou três camadas de células poliédricas; o estrato granuloso não foi observado nos exemplares estudados O estrato córneo apresenta uma delgada camada de queratina mole. Na derme, os fibroblastos foram as células mais freqüentes e as fibras colágenas formavam feixes espessos dispostos em várias direções. No método do Picro Sirius Red sob luz polarizada observou-se que, independente da região analisada, há predomínio de fibras colágenas tipo I em relação ao colágeno tipo III. Foi também observados mastócitos em pequena quantidade e fibras elásticas na região subepidérmica. Concluiu-se que a pele de Kinosternon scorpioides scorpioides possui características semelhantes a dos demais vertebrados (anfíbios, aves e mamíferos), apresenta peculiaridades, como por exemplo, a ausência de papilas dérmicas e glândulas...

The aim of this paper was to study the histology and histochemistry of the skin of six specimens of muçuã (three males and three females). The animals were euthanized through a lethal dose of sodium thiopental at 2.5%. Fragments of the soft skin were fixed in Bouin's solution and processed for inclusion in paraffin. The sections were stained with hematoxylin-eosin, Giemsa, Sirius red, Gomori's reticulin and Weigert's fuchsin-resorcin. The results revealed that the skin is thin and constituted by epidermis and dermis. The epidermis is made up by one layer of cylindrical cells of stratum germinativum, two or three layers of poliedric cells of stratum spinosum, but without stratum granular. The stratum corneum consists of a thin layer of soft keratin. In the dermis, the fibroblasts were the most frequent cells and collagen fibers formed a thick bound displayed in several directions. The Sirius red under polarized light showed that type I collagen was predominant when compared with the occurrence of type III collagen. Mast cells were also found, and elastic fibers were seen in the subepidermic layer. We concluded that the skin of Kinosternon scorpioides scorpioides has histological features similar to other vertebrates (amphibia, aves, mammalia), however without dermal papillae and glands...

Comparação da contenção farmacológica com cetamina e xilazina, administradas pela via intramuscular no membro torácico ou pélvico, em jacarés-do-papo-amarelo juvenis / Comparison of pharmacological restraint with ketamine and xylazine, administered intramuscularly in the forelimb or hindlimb, in broad-snouted caiman juveniles

Os répteis possuem um sistema porta-renal, o qual pode desviar parte do sangue proveniente das porções caudais do corpo aos rins antes que a mesma atinja a circulação sistêmica. Em vista disto, vem sendo aconselhada a administração de medicamentos injetáveis nos membros torácicos, para que se evite a filtração imediata pelo parênquima renal, causando redução do efeito esperado. O objetivo do presente estudo foi comparar aspectos qualitativos e quantitativos da associação de cetamina (30 mg/kg) e xilazina (1 mg/kg), injetada no membro torácico ou pélvico, em jacarés-do-papo-amarelo (Caiman latirostris) juvenis. Oito animais machos com peso médio (±DP) de 1,3 (±0,3) kg e, aproximadamente, dois anos de idade foram anestesiados em duas ocasiões distintas com intervalo de sete dias. Em cada ocasião, os animais receberam, de forma aleatória, a associação anestésica por via intramuscular em membro torácico (tratamento MT) ou pélvico (tratamento MP). Foram avaliados os intervalos de tempo entre a administração do tratamento e a perda do reflexo de endireitamento (período de indução), entre a perda e o retorno desse reflexo (duração do efeito clínico importante) e entre o retorno do reflexo de endireitamento e os primeiros movimentos de deambulação (duração do efeito residual), as frequências cardíaca e respiratória e as temperaturas ambiental e cloacal. Os escores de sedação/anestesia foram avaliados através de uma escala com variação de 0 (alerta/consciente) a 10 (anestesia profunda/sobredosagem). No tratamento MP, dois animais não apresentaram perda de reflexo de endireitamento. Considerando somente aqueles que apresentaram a perda desse reflexo, o tempo de indução (21±9 e 17±5 minutos) e a duração do efeito clínico importante (35±19 e 43±21 minutos) e residual (28±31 e 12±11 minutos) foram similares entre os tratamentos MT e MP (média±desvio padrão)...

Reptiles possess a renal portal system which can divert part of the blood from the caudal portions of the body to the kidney before it reaches the systemic circulation. In view of this, it has been recommended the administration of injectable medications in the forelimbs, in order to avoid immediate glomerular filtration, which might result in a reduction of the expected effect. The aim of this study was to compare qualitative and quantitative aspects of the pharmacological restraint provided by the combination of ketamine (30mg/kg) and xylazine (1mg/kg), injected into the forelimb or hindlimb, in broad-snouted caiman juveniles (Caiman latirostris). Eight male animals, with a mean weight (±SD) of 1.3 (±0.3) kg, and aged about 2 years old, were anesthetized on two separate occasions with an interval of 7 days. On each occasion, the animals were randomly assigned to receive the anesthetic combination intramuscularly into the forelimb (FL treatment) or hindlimb (HL treatment). The time intervals between administration of treatment and loss of the righting reflex (induction time), between the loss and return of this reflex (duration of important clinical effect), and between the return of the righting reflex and first movements of ambulation (duration of residual effect) were measured as well as heart and respiratory rates and cloacal and environmental temperatures. Sedation/anesthesia scores were evaluated using a scale ranging from 0 (alert/conscious) to 10 (deep anesthesia/overdose). In the HL treatment, loss of righting reflex was not observed in two animals. Considering only those animals whose loss of righting reflex was observed, the induction time (21±9 and 17±5 minutes), the duration of important clinical effect (35±19 and 43±21 minutes), and the duration of residual effect (28±31 and 12±11 minutes) were similar between the FL and HL treatments, respectively (mean±SD). Sedation/anesthesia scores were significantly higher than at baseline...

A cadaveric study to determine the minimum volume of methylene blue to completely color the nerves of brachial plexus in cats. An update in forelimb and shoulder surgeries

PURPOSE: To determine the minimum volume of methylene blue (MB) to completely color the brachial plexus (BP) nerves, simulating an effective anesthetic block in cats. METHODS: Fifteen adult male cat cadavers were injected through subscapular approach with volumes of 2, 3, 4, 5 and 6 ml in both forelimbs, for a total of 30 brachial plexus blocks (BPB). After infusions, the specimens were carefully dissected preserving each nervous branch. The measurement of the effective area was indicated by the impregnation of MB. Nerves were divided into four segments from the origin at the spinal level until the insertion into the thoracic limb muscles. The blocks were considered effective only when all the nerves were strongly or totally colored. RESULTS: Volumes of 2, 3 and 4 ml were considered insufficient suggesting a failed block, however, volumes of 5 and 6 ml were associated with a successful block. CONCLUSIONS: The injection of methylene blue, in a volume of 6 ml, completely colored the brachial plexus. At volumes of 5 and 6 ml the brachial plexus blocks were considered a successful regional block, however, volumes of 2, 3 and 4 ml were considered a failed regional block. .

Enhanced Efficacy of Human Brain-Derived Neural Stem Cells by Transplantation of Cell Aggregates in a Rat Model of Parkinson's Disease

OBJECTIVE: Neural tissue transplantation has been a promising strategy for the treatment of Parkinson's disease (PD). However, transplantation has the disadvantages of low-cell survival and/or development of dyskinesia. Transplantation of cell aggregates has the potential to overcome these problems, because the cells can extend their axons into the host brain and establish synaptic connections with host neurons. In this present study, aggregates of human brain-derived neural stem cells (HB-NSC) were transplanted into a PD animal model and compared to previous report on transplantation of single-cell suspensions. METHODS: Rats received an injection of 6-OHDA into the right medial forebrain bundle to generate the PD model and followed by injections of PBS only, or HB-NSC aggregates in PBS into the ipsilateral striatum. Behavioral tests, multitracer (2-deoxy-2-[18F]-fluoro-D-glucose ([18F]-FDG) and [18F]-N-(3-fluoropropyl)-2-carbomethoxy-3-(4-iodophenyl)nortropane ([18F]-FP-CIT) microPET scans, as well as immunohistochemical (IHC) and immunofluorescent (IF) staining were conducted to evaluate the results. RESULTS: The stepping test showed significant improvement of contralateral forelimb control in the HB-NSC group from 6-10 weeks compared to the control group (p<0.05). [18F]-FP-CIT microPET at 10 weeks posttransplantation demonstrated a significant increase in uptake in the HB-NSC group compared to pretransplantation (p<0.05). In IHC and IF staining, tyrosine hydroxylase and human beta2 microglobulin (a human cell marker) positive cells were visualized at the transplant site. CONCLUSION: These results suggest that the HB-NSC aggregates can survive in the striatum and exert therapeutic effects in a PD model by secreting dopamine.

The treatment of lower extremity defects with severe proliferative tissue using an adjustable horizontal mattress suture in a Tosa dog / 대한수의학회지

A 2-year-old male Tosa was admitted for treatment of a non-healing wound on the right forelimb. Skin tests were unremarkable; however, the lesion contained severe proliferative tissue. Surgical treatment was conducted due to the extensive skin defect and granulation tissue present. Following removal of the proliferative tissue, the wound was closed using the adjustable horizontal mattress suture method with multiple punctate relaxing incisions. The proliferative tissue healed completely after the surgical treatment. This technique can be considered an alternative treatment for the proliferative tissue when conditions require a skin graft or flap after surgical treatment.